• 经测试应用WB, IP, Flow Cytmore details
• 种属反应性
  与反应: Mouse, Rat, Human
• 免疫原

  Recombinant fragment within Human 2 Hydroxy phytanoyl CoA lyase aa 200-400. The exact sequence is proprietary.
  Database link: Q9UJ83

  Run BLAST with Run BLAST with 
• 阳性对照
  • HeLa, 293T, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse brain, Mouse heart, Rat brain and Rat heart lysates; 293T cells; HeLa cells.
• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
• 浓度100 µl 浓度为 0.854 mg/ml 40 µl 浓度为 0.854 mg/ml 10 µl 浓度为 0.854 mg/ml 
  
• 纯度Protein A purified
• 克隆单克隆
• 克隆编号EPR16812
• 同种型IgG
• 研究领域
  • Tags & Cell Markers
   
  • Subcellular Markers
   
  • Organelles
   
  • Peroxisome

  • Cancer
   
  • Cancer Metabolism
   
  • Metabolic signaling pathway
  • Metabolism of lipids and lipoproteins

  • Metabolism
   
  • Pathways and Processes
   
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
   
  • Lipid metabolism

应用

靶标

• 相关性2 Hydroxy phytanoyl CoA lyase catalyzes a carbon-carbon cleavage reaction. It cleaves a 2 hydroxy 3 methylacyl CoA into formyl CoA and a 2 methyl branched fatty aldehyde.
• 细胞定位Peroxisome
• 数据库链接
  • Entrez Gene: 26061 Human
  • Entrez Gene: 56794 Mouse
  • Entrez Gene: 85255 Rat
  • Omim: 604300 Human
  • SwissProt: Q9UJ83 Human
  • SwissProt: Q9QXE0 Mouse
  • SwissProt: Q8CHM7 Rat
  • Unigene: 63290 Human

  see all
• 别名
  • 1600020H07Rik antibody
  • 2 HPCL antibody
  • 2 hydroxyacyl CoA lyase 1 antibody

  see all

Anti-2 Hydroxy phytanoyl CoA lyase antibody [EPR16812] 图像

• 

  Western blot - Anti-2 Hydroxy phytanoyl CoA lyase antibody [EPR16812] (ab197025)
  All lanes : Anti-2 Hydroxy phytanoyl CoA lyase antibody [EPR16812] (ab197025) at 1/1000 dilution
  
  Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
  Lane 2 : 293T (Human epithelial cells from embryonic kidney) whole cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
  
  Predicted band size : 64 kDa
  Observed band size : 64 kDa
  
  
  Exposure time : 15 seconds

  Blocking/Dilution buffer: 5% NFDM/TBST.

• 

  Western blot - Anti-2 Hydroxy phytanoyl CoA lyase antibody [EPR16812] (ab197025)
  All lanes : Anti-2 Hydroxy phytanoyl CoA lyase antibody [EPR16812] (ab197025) at 1/1000 dilution
  
  Lane 1 : Mouse brain lysate
  Lane 2 : Mouse heart lysate
  Lane 3 : Rat brain lysate
  Lane 4 : Rat heart lysate
  Lane 5 : C6 (Rat glial tumor cells) whole cell lysates
  Lane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
  Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
  Lane 8 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
  
  Predicted band size : 64 kDa
  Observed band size : 64 kDa
  
  
  Exposure time : 1 minute

  Blocking/Dilution buffer: 5% NFDM/TBST.

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