Abcam’s 11,12 DHET in vitro competitive ELISA kit is designed for accurate quantitative measurement in serum, plasma, urine, cell culture and tissue samples following proper isolation and purification.
This competitive ELISA kit is based on competition between the 11,12 DHET epitope and the 11,12 DHET-HRP conjugate for a limited number of binding sites available from the anti-11,12 DHET antibody; which is coated to the wells of the 96 well ELISA plate. The conjugate concentration is held as a constant in each well, while the concentration of the 11,12 DHET is variable, based on the concentration of the sample or standard. Thus the amount of the 11,12 DHET conjugate which is able to bind to each of the wells is inversely proportional to the concentration of 11,12 DHETin the standard or sample. The amount of the conjugate which is bound to each well is then determined by the amount of color obtained, when TMB is added. The TMB reacts with the HRP available in the well. With the addition of sulfuric acid, the blue colored product is converted into a yellow colored product, which can be read on a plate reader at 450 nm.
The level of 11,12-DHET or 11,12-DHET epitope has been shown to exhibit correlation with hypertension in rodents (1,2,3). 11,12-DHET is a representative metabolite of cytosolic epoxide hydrolase-mediated metabolism of EETs, which are generated by arachidonic acid epoxygenase activity of cytochrome P450’s (4).
