pet11c载体基本信息
出品公司:别名:质粒类型:表达水平:克隆方法:载体大小:5' 测序引物:5' 测序引物序列:  载体标签:载体抗性:备注:产品目录号:稳定性:组成型:病毒/非病毒:
EMD Biosciences(Novagen)
pET11c, pet 11c
大肠杆菌蛋白表达
高
多克隆位点，限制性内切酶
5672bp
T7
5'-TAATACGACTCACTATAGGG-3'
N-T7
Ampicillin
Approximate size is 5675 bp. Hosts: E.coli. Related vectors: pBR322.
69438-3
瞬时表达 Transient
组成型 Constitutive
非病毒
pet11c载体质粒图谱和多克隆位点信息


Feature Name 
Start 
End 
 
T7_terminator
316
188
T7_Terminal_primer
256
274
T7_leader
358
326
Xpress_fwd_primer
359
341
T7_transl_en_RBS
383
367
lacO
428
401
T7_promoter
446
428
pBRrevBam_primer
549
530
lacI
824
1915
tet (576 - 1190)
1974
2588
ROP
3227
3418
pGEX_3_primer
3434
3412
Ampicillin
5464
4604
AmpR_promoter
5534
5506
ORF 
Start 
End 
 
ORF frame 1
583
1083
ORF frame 2
956
1915
ORF frame 1
2393
1698
ORF frame 3
1971
2588
ORF frame 2
5464
4604
Enzyme Name 
Cut
ClaI
24
HindIII
29
BamHI
319
NheI
350
NdeI
357
XbaI
395
BglII
461
BclI
1197
ApaI
1394
HpaI
1689
EagI
2251
NruI
2286
MscI
2758
PstI
4922
AatII
5599
EcoRI
5670
pet11c载体简介
The pET-11a-d vectors carry an N-terminal T7•Tag® sequence and BamH I cloning site. Thesevectors are the precursors to many pET family vectors; the pET-21a-d(+) series corresponds topET-11a-d but incorporates several additional features. Unique sites are shown on the circle map.Note that the sequence is numbered by the pBR322 convention, so the T7 expression region isreversed on the circular map. The cloning/expression region of the coding strand transcribed byT7 RNA polymerase is shown below.
pet11c载体序列

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