BioScript All-in-One cDNA Synthesis SuperMix

Advantages

Convenience: One tube provides all components necessary for reverse transcription.
Reproducibility: Modified Oligo dT: Random primers ratio facilitates qPCR repeatability.
High compatibility: cDNA product is suitable for application with SYBR Green or Probe qPCR.

Price Comparison

Brand	Price/rxn	Product form	Enzyme reaction temperature	RT extension time
Biotool	0.45 USD	All-in-One	42°C	15-30 min
Applied Biotechnology	0.78 USD	Multi-Step	37°C	120 min
Bio-Rad	4.65 USD	All-in-One	42°C	30 min

Description

BioScript All-in-One cDNA Synthesis SuperMix provides first-strand cDNA synthesis, optimized with the high-temperature capability of Reverse Transcriptase and an optimized SuperMix formula. In one tube, the pre-blended 5× qRT SuperMix contains all the necessary components for reverse transcription of YOUR RNA template. The resulting cDNA product is suitable for use in real-time quantitative RT-PCR (qRT-PCR).

Kit Components

Component	B24403	B24408
5×qRT SuperMix *	400 μL	1 mL × 2
5 × No RT ControlMix ♥	40 μL	200 μL
RNase-free Water	1 mL	1mL × 5
* contains Buffer, dNTP, Reverse Transcriptase, RNase inhibitor, Oligo dT/Random primer mix. ♥ contains all the components in 5 × qRT SuperMix, except Reverse Transcriptase.

Application

Synthesis of cDNA from target genes for qRT-PCR, especially low-copy genes or RNA templates of low concentration.

Storage

Store at -20°C for up to one year. Repetitive freeze-thaw cycles should be avoided. It is recommended to pre-aliquot the mix into small batches for frequent usage. Product quality is guaranteed under proper storage conditions.

Caution

For Research Use Only! Not intended for any therapeutic or diagnostic procedures!

更多详情请访问唯一官方网站http://www.biotool.cn/product/bioscript-all-in-one-cdna-synthesis-supermix.html