重组人GITR蛋白（Recombinant Human GITR Protein），GMP级

产品货号：GMP-TL720

产品描述（Description）

Glucocorticoid-induced TNFR-related protein (GITR) is also known as Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), activation-inducible TNFR family receptor (AITR), CD antigen CD357, which is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is receptor for TNFSF18, which seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. GITR also mediated NF-kappa-B activation via the TRAF2/NIK pathway.

分子别名（Synonym）

AITR、TNFRSF18、CD357

表达宿主（Expression host）

HEK293细胞

来源（Source）

Human

蛋白序列（Protein Sequence）

DNA序列编码人GITR（NCBI Reference Sequence:NP_683700.1）表达带有Fc标签在C末端

分子量（Molecular Weight）

重组人GITR蛋白 包含368个氨基酸，预测理论分子量为41.5kD

纯度（Purity）

≥95%采用SDS-PAGE凝胶和高效液相色谱分析

内毒素（Endotoxin）

＜0.1EU/ug

生物活性（Biological Activity）

固定人 GITR(Fc 标签)5ug/mL(100uL/孔)能结合人 GITR Ligand（His 标签)的线性范围是 2-100 ng/mL

稳定性（Stability）

冻干的样本的可以在4℃保存24个月，溶解后的液体可以于-20℃保存6-12个月，并且避免反复冻融

组成（Component）

含有6%甘露醇的无菌PBS（PH 7.4）的冻干粉

参考文献

1、GITR Intrinsically Sustains Early Type 1 and Late Follicular Helper CD4 T Cell Accumulation to Control a Chronic Viral Infection Derek L. Clouthier, Angela C. Zhou, Michael E. Wortzman, Olga Luft, Gary A. Levy, Tania H. Watts PLoS Pathog. 2015 Jan; 11(1): e1004517. Published online 2015 Jan 15. doi: 10.1371/journal.ppat.1004517 

2、Novel Tumor Suppressor Function of Glucocorticoid-Induced TNF Receptor GITR in Multiple Myeloma Yang Liu, Phong Quang, Esteban Braggio, Hai Ngo, Gayane Badalian-Very, Ludmila Flores, Yong Zhang, Antonio Sacco, Patricia Maiso, Abdel Kareem Azab, Feda Azab, Ruben Carrasco, Barrett J. Rollins, Aldo M. Roccaro, Irene M. Ghobrial PLoS One. 2013; 8(6): e66982. Published online 2013 Jun 13. doi: 10.1371/journal.pone.0066982 

3、Expansion of regulatory GITR+CD25low/-CD4+ T cells in systemic lupus erythematosus patientsGiuseppe Nocentini, Alessia Alunno, Maria Grazia Petrillo, Onelia Bistoni, Elena Bartoloni, Sara Caterbi, Simona Ronchetti, Graziella Migliorati, Carlo Riccardi, Roberto Gerli Arthritis Res Ther. 2014; 16(5): 444. Published online 2014 Sep 26. doi: 10.1186/s13075-014-0444-x

  注意：For research and manufacturing use.