重组人ICOS蛋白（Recombinant Human ICOS Protein），GMP级

产品货号：GMP-TL723

产品描述（Description）

Inducible T-cell costimulator (ICOS) is also known as Activation-inducible lymphocyte immunomediatory molecule (AILIM), CD278, which belongs to the CD28 family of immune costimulatory receptors consisting of CD28, CTLA-4 and PD-1. ICOS enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. CD278 / ICOS prevents the apoptosis of pre-activated T-cells and also plays a critical role in CD40-mediated class switching of immunoglobin isotypes.

分子别名（Synonym）

CD278、AILIM、Inducible T-cell costimulator

表达宿主（Expression host）

HEK293细胞

来源（Source）

Human

蛋白序列（Protein Sequence）

DNA序列编码人ICOS（NCBI Reference Sequence: NP_036224.1）表达带有Fc标签在C末端

分子量（Molecular Weight）

重组人ICOS蛋白 包含354个氨基酸，预测理论分子量为40.7kD

纯度（Purity）

≥95%采用SDS-PAGE凝胶和高效液相色谱分析

内毒素（Endotoxin）

＜0.1EU/ug

生物活性（Biological Activity）

固定人 B7-H2(His 标签)2ug/mL (100uL/孔) 能够结合 ICOS(Fc 标签)的线性范围是 0.2-10 ng/mL

稳定性（Stability）

冻干的样本的可以在4℃保存24个月，溶解后的液体可以于-20℃保存6-12个月，并且避免反复冻融

组成（Component）

含有6%甘露醇的无菌PBS（PH 7.4）的冻干粉

参考文献

1、Differentiation of ICOS+ and ICOS− recent thymic emigrant regulatory T cells (RTE Tregs) during normal pregnancy, pre‐eclampsia and HELLP syndrome M. I. Wagner, M. Jöst, J. Spratte, M. Schaier, K. Mahnke, S. Meuer, M. Zeier, A. Steinborn Clin Exp Immunol. 2016 Jan; 183(1): 129–142. Published online 2015 Oct 12. doi: 10.1111/cei.12693 

2.、ICOS Co-Stimulation: Friend or Foe? Daniel J. Wikenheiser, Jason S. Stumhofer Front Immunol. 2016; 7: 304. Published online 2016 Aug 10. doi: 10.3389/fimmu.2016.00304 

3、Loss of Immune Tolerance Is Controlled by ICOS in Sle1 MiceNanette Mittereder, Ellen Kuta, Geetha Bhat, Karma Dacosta, Lily I. Cheng, Ronald Herbst, Gianluca Carlesso J Immunol. 2016 Jul 15; 197(2): 491–503. Published online 2016 Jun 13. doi: 10.4049/jimmunol.1502241 

4、Development of interleukin-17-producing Vγ2+ γδ T cells is reduced by ICOS signaling in the thymus Terkild Brink Buus, Jonas Damgård Schmidt, Charlotte Menné Bonefeld, Carsten Geisler, Jens Peter Holst Lauritsen Oncotarget. 2016 Apr 12; 7(15): 19341–19354. Published online 2016 Mar 29. doi: 10.18632/oncotarget.8464

  注意：For research and manufacturing use.