• 形式Liquid
• 存放说明Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at +4°C. Do Not Freeze.
• 存储溶液pH: 7.4
  Preservative: 0.05% Sodium azide
  Constituents: PBS, Sodium phosphate, Sodium chloride
•  
• 纯度Purified via His tag
• 纯化说明This product is a recombinant protein produced in E. coli.
• 克隆单克隆
• 常规说明

  Sepharose® is a registered trademark of GE Healthcare.

• 研究领域
  • Secondary antibodies
   
  • anti-Rabbit
   
  • IgG
   
  • Enzyme
   
  • HRP

应用

Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) 图像

• 

  Immunoprecipitation - sdAb anti-Rabbit IgG Agarose (ab191867)

  Lane 1: Diluted Rabbit Sera (1 in 100)

  Lane 2: Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) (ab191867) + Rabbit Sera

  Lane 3: Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) (ab191867) IP Flow-through

  Lane 4:  Blocked beads + Rabbit Sera

  Lane 5: Diluted Rabbit Sera (1 in 100)

  Lane 6: Protein A beads + Rabbit Sera

  Lane 7: Protein A beads IP Flow-through

   

  Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) ab191867 beads were used to pull down Rabbit IgG from Rabbit Sera. Protein A beads were used as a positive control and blocked beads as a negative. The gel was stained with Optiblot Blue (ab119211).

• 

  Immunoprecipitation - sdAb anti-Rabbit IgG Agarose (ab191867)

  Lane 1: 2µg HeLa Lysate

  Lane 2: 2µg Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) IP supernatant

  Lane 3: Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) (ab191867) pulling down B-tubulin (50kDa)

  Lane 4: Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) (ab191867) no IP ab, Negative Control

  Lane 5: 2µg HeLa Lysate

  Lane 6: 2µg Protein A beads IP supernatant

  Lane 7: Protein A beads pulling down B-tubulin (50kDa)

   

  Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) (ab191867) beads were used to pull down β-tubulin from HeLa cell lysate with rabbit anti-β-tubulin (ab6046). Protein A agarose beads were used as a positive control. The gel was transferred to Nitrocellulose membrane and probed with mouse anti-β-tubulin (ab101019) and anti-mouse HRP (ab97040).

• 

  Immunoprecipitation - sdAb anti-Rabbit IgG Agarose (ab191867)

  Lane 1: Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) (ab191867) + 50µg Rabbit IgG Control (ab46540)

  Lane 2: Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) (ab191867) IP Supernatant
  Lane 3: Protein A beads + 50µg Rabbit IgG Control (ab46540)
  Lane 4: Protein A beads IP Supernatant

   

  Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) ab191867 beads were used to pull down Rabbit IgG Control, 50µg (ab46540). Protein A beads were used as a positive control. The gel was stained with Optiblot Blue (ab119211).

• 

  Immunoprecipitation - sdAb anti-Rabbit IgG Agarose (ab191867)

  Lane 1: 2µg HeLa Lysate
  Lane 2: Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) (ab191867) beads IP supernatant
  Lane 3: Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) (ab191867) pulling down GAPDH from HeLa lysate (37 kDa)
  Lane 4: Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) (ab191867), no IP ab (negative control)
  Lane 5: 2µg HeLa Lysate
  Lane 6: Protein A beads IP supernatant
  Lane 7: Protein A beads pulling down GAPDH from HeLa lysate (37 kDa)

   

  Anti-Rabbit IgG VHH Single Domain Antibody (Sepharose) (ab191867) beads were used to pull down GAPDH from HeLa cell lysate with rabbit anti-GAPDH (ab128915). Protein A agarose beads were used as a positive control. The gel was transferred to Nitrocellulose membrane and probed with mouse anti-GAPDH (ab8245) and anti-mouse HRP (ab97040).